To investigate effects of diets with different fatty acid composition on serum lipid profiles, inflammation, oxidative stress and endothelial function in mice fed high-fat diets.

METHODS: Male KM mice were randomly divided into five groups and were fed normal control diet, high-fat lard diet, high-fat diets with n-6/n-3 polyunsaturated fatty acid (PUFA) ratios of 1:1, 5:1 nd 2:1 for fiv weeks, respectively. The levels of serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C ) iterlekin-6 (IL-6), alonildehyde (MDA), hypersensitive C-reactive protein (hs-CRP), tumor ncrosis factor alpha (TNF-alpha), lipid lphaproxide (LPO), 8-iso postaglandin F2aalpha (8-iso-PGlphaF2u), oxidialphaed low-density lipoprotein (ox-LDL), free faty acid (FFA), E-selectin (ES) and von Willebrand factor (vWF) were measured.

RESULTS: The levels of serum LDL-C and non-HDL-C in the lard group were significantly higher than those in the other groups (P < 0.05). Th e ard group had. significntly higher serum TG and TC concentrations compared to 1:1 and 5: groups P <0. 05). The evels of serum FFA in 20:1 group wre significantly higher than those in 1:1 and 5:1 groups P < 0.05). Co mpred with the lard and 20:1 groups,the 1:1 and 5:1 groups displayed lower levels of inflammatory cytokines, oxidative stress and ES. The 5:1 group sgnificantly decreased the level of serum vWF compared to lard and 20:1 groups P < 0.05).

CONCLUSION: diet with low n-6/n-3 PUFA ratio could improve lipid metabolism, inflammation, oxidative stress and endothelial function compared to high-fat diets with lard and higher n-6/n-3 PUFA ratio. The diet with low n-6/n-3 PUFA ratio can improve cardiovascular disease risk factors to prevent cardiovascular disease